Freezing Induces Biased Results in the Molecular Detection of Flavobacterium columnare-Reference-Cited by-同舟云学术

Freezing Induces Biased Results in the Molecular Detection of Flavobacterium columnare

Published:2006-02 Issue:2 Volume:72 Page:1702-1704
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Suomalainen Lotta-Riina¹,Reunanen Hilkka¹,Ijäs Ritva¹,Valtonen E. Tellervo¹,Tiirola Marja¹

Affiliation:

1. Department of Biological and Environmental Science, University of Jyväskylä, P.O. Box 35, FIN-40014 University of Jyväskylä, Finland

Abstract

ABSTRACT Specific PCR detection and electron microscopy of Flavobacterium columnare revealed the risk of false-negative results in molecular detection of this fish pathogen. Freezing and thawing destroyed the cells so that DNA was for the most part undetectable by PCR. The detection of bacteria was also weakened after prolonged enrichment cultivation of samples from infected fish.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.72.2.1702-1704.2006

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