Detection of Enterococcal Surface Protein Gene ( esp ) and Amplified Fragment Length Polymorphism Typing of Glycopeptide-Resistant Enterococcus faecium during Its Emergence in a Greek Intensive Care Unit

Author:

Routsi C.1,Platsouka E.2,Willems R. J. L.3,Bonten M. J. M.4,Paniara O.2,Saroglou G.1,Roussos C.1

Affiliation:

1. Department of Critical Care, Medical School of Athens University

2. Department of Clinical Microbiology, Evangelismos Hospital, Athens, Greece

3. Diagnostic Laboratory for Infectious Diseases and Perinatal Screening, National Institute of Public Health and the Environment, Bilthoven

4. Department of Internal Medicine, University Medical Center, Utrecht, The Netherlands

Abstract

ABSTRACT The emergence of glycopeptide-resistant Enterococcus faecium (GREF) in a Greek intensive care unit was studied by amplified fragment length polymorphism analysis and esp gene detection. Three GREF clones harboring the esp gene were recovered from 17 out of 21 patients, indicating the dissemination of genetically homogenous and virulent strains of GREF.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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