Rapid Enterovirus RNA Detection in Clinical Specimens by Using Nucleic Acid Sequence-Based Amplification-Reference-Cited by-同舟云学术

Rapid Enterovirus RNA Detection in Clinical Specimens by Using Nucleic Acid Sequence-Based Amplification

Published:2003-01 Issue:1 Volume:41 Page:346-350
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Landry Marie L.¹²,Garner Robin²,Ferguson David¹²

Affiliation:

1. Department of Laboratory Medicine, Yale University School of Medicine, New Haven, Connecticut 06520

2. Clinical Virology Laboratory, Yale New Haven Hospital, New Haven, Connecticut 06504

Abstract

ABSTRACT Enterovirus (EV) detection by nucleic acid sequence-based amplification was compared with EV isolation in cell culture. The NucliSens Basic kit (bioMerieux) was utilized for RNA detection. For virus isolation, samples were inoculated into MRC-5, primary rhesus monkey kidney, A549, rhabdomyosarcoma, and/or Buffalo green monkey kidney cells.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.41.1.346-350.2003

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