Measurement of Epstein-Barr Virus DNA Loads in Whole Blood and Plasma by TaqMan PCR and in Peripheral Blood Lymphocytes by Competitive PCR-Reference-Cited by-同舟云学术

Measurement of Epstein-Barr Virus DNA Loads in Whole Blood and Plasma by TaqMan PCR and in Peripheral Blood Lymphocytes by Competitive PCR

Published:2003-11 Issue:11 Volume:41 Page:5245-5249
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Wadowsky Robert M.¹²³,Laus Stella³,Green Michael⁴⁵³,Webber Steven A.⁴³,Rowe David²

Affiliation:

1. Departments of Pathology

2. Department of Infectious Diseases and Microbiology, Graduate School of Public Health, University of Pittsburgh

3. Children's Hospital of Pittsburgh, Pittsburgh, Pennsylvania 15213

4. Pediatrics

5. Surgery, School of Medicine

Abstract

ABSTRACT Epstein-Barr virus (EBV) DNA load values were measured in samples of whole blood ( n = 60) and plasma ( n = 59) by TaqMan PCR and in samples of peripheral blood lymphocytes (PBLs) ( n = 60) by competitive PCR (cPCR). The samples were obtained from 44 transplant recipients. The whole-blood and PBL loads correlated highly ( r 2 > 0.900), whereas the plasma and PBL loads correlated poorly ( r 2 = 0.512). Testing of whole blood by TaqMan PCR is an acceptable alternative to testing of PBLs by cPCR for quantifying EBV DNA load.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.41.11.5245-5249.2003

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