Differentiation of Mycoplasma Species by 16S Ribosomal DNA PCR and Denaturing Gradient Gel Electrophoresis Fingerprinting-Reference-Cited by-同舟云学术

Differentiation of Mycoplasma Species by 16S Ribosomal DNA PCR and Denaturing Gradient Gel Electrophoresis Fingerprinting

Published:2003-10 Issue:10 Volume:41 Page:4844-4847
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

McAuliffe Laura¹,Ellis Richard J.²,Ayling Roger D.¹,Nicholas Robin A. J.¹

Affiliation:

1. Mycoplasma Group, Department of Bacterial Diseases, Veterinary Laboratories Agency (Weybridge), Surrey, KT15 3NB

2. NERC Centre for Population Biology, Imperial College London, Silwood Park Campus, Ascot, Berks, SL5 7PY, United Kingdom

Abstract

ABSTRACT Denaturing gradient gel electrophoresis (DGGE) of a 16S ribosomal DNA PCR product was used to differentiate 32 mycoplasma species of veterinary significance. Twenty-seven (85%) species could be differentiated by DGGE. This method could enable the rapid identification of many mycoplasma species for which there is no specific PCR available and which are currently identified by using culture and serological tests.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.41.10.4844-4847.2003

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