Uses and Limitations of the XTT Assay in Studies of Candida Growth and Metabolism-Reference-Cited by-同舟云学术

Uses and Limitations of the XTT Assay in Studies of Candida Growth and Metabolism

Published:2003-01 Issue:1 Volume:41 Page:506-508
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Kuhn D. M.¹²,Balkis M.²,Chandra J.²,Mukherjee P. K.²,Ghannoum M. A.²

Affiliation:

1. Division of Infectious Diseases, Department of Medicine, Case Western Reserve University

2. Center for Medical Mycology, Department of Dermatology, University Hospitals of Cleveland and Case Western Reserve University, Cleveland, Ohio 44106

Abstract

ABSTRACT Colorimetric tetrazolium assays are used increasingly in studies of fungi, often in the absence of standardization or correlation with other methods. We examined species- and strain-related tetrazolium metabolism in Candida albicans and Candida parapsilosis by using XTT {2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide} and WST-8 [2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulphonyl)-2H-tetrazolium] and found marked variations. Also, significant signal was often missed in the absence of dimethyl sulfoxide extraction.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.41.1.506-508.2003

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