Real-Time PCR Assay for Clinical Management of Human Immunodeficiency Virus-Infected Patients with Visceral Leishmaniasis-Reference-Cited by-同舟云学术

Real-Time PCR Assay for Clinical Management of Human Immunodeficiency Virus-Infected Patients with Visceral Leishmaniasis

Published:2003-11 Issue:11 Volume:41 Page:5080-5084
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Bossolasco Simona¹,Gaiera Giovanni¹,Olchini Davide¹,Gulletta Maurizio²,Martello Leonardo¹,Bestetti Arabella¹,Bossi Laura³,Germagnoli Luca⁴,Lazzarin Adriano¹,Uberti-Foppa Caterina¹,Cinque Paola¹

Affiliation:

1. Clinic of Infectious Diseases

2. Department of Infectious Disease and Tropical Medicine, Spedali Civili, Brescia, Italy

3. Central Laboratory Analysis, Ospedale Maggiore, IRCCS, Milan

4. Central Laboratory Analysis, San Raffaele Hospital

Abstract

ABSTRACT To evaluate the usefulness of a real-time PCR for Leishmania DNA in the diagnosis and follow-up of patients with human immunodeficiency virus type 1 (HIV-1) and Leishmania coinfection, Leishmania DNA levels were measured in whole peripheral blood from 25 HIV-infected patients with clinical features suggestive of visceral leishmaniasis. Leishmania DNA was detected in 10 of 25 patients with microscopically confirmed visceral leishmaniasis and in none of those without this disease. Following treatment with liposomal amphotericin B, a clinical response was observed in 9 of 10 patients, in association with significantly decreased parasite loads. Seven patients relapsed clinically a median of 110 days after the end of treatment, in association with substantial increases in Leishmania DNA levels. Leishmania DNA levels correlated with the clinical course of visceral leishmaniasis, and their measurement at diagnosis and during and after treatment seems to be useful in the clinical management of HIV-infected patients with this disease.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.41.11.5080-5084.2003

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