Affiliation:
1. Division of Biological and Medical Research, Argonne National Laboratory, Argonne, Illinois 60439
Abstract
A photocell-controlled continuous culture device, a Nephelostat, is described that maintains a wide variety of cultures of microorganisms in balanced growth. This Nephelostat controls concentrations of bacteria within ±3% over a cell concentration range of 10
6
to 10
9
cells per ml. Growth rates are recorded so that changes in the growth rate are observed over small increments of time. Spontaneous and caffeine-induced mutation rates of two strains of
Escherichia coli
were compared under Nephelostat and chemostat conditions.
Publisher
American Society for Microbiology
Subject
General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine
Cited by
4 articles.
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