Isolation of Vi Antigen and a Simple Method for Its Measurement-Reference-Cited by-同舟云学术

Isolation of Vi Antigen and a Simple Method for Its Measurement

Published:1972-10 Issue:4 Volume:24 Page:628-633
ISSN:0003-6919
Container-title:Applied Microbiology
language:en
Short-container-title:Appl Microbiol

Author:

Wong K. H.¹,Feeley John C.¹

Affiliation:

1. Laboratory of Bacterial Products, Division of Biologics Standards, National Institutes of Health, Bethesda, Maryland 20014

Abstract

Vi antigen was purified from saline extract of Citrobacter strain 5396/38 by enzymatic digestion, concentration with ethanol, and precipitation with hexadecyltrimethylammonium bromide. The Vi antigen prepared by this method was 18 and 230 times more protective in mice than two other preparations isolated by an earlier method utilizing acid hydrolysis. A sensitive and specific method of electroimmunodiffusion was described for measurement of Vi antigen.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Link

https://journals.asm.org/doi/pdf/10.1128/am.24.4.628-633.1972

Reference22 articles.

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