Rapid determination of minimum inhibitory concentrations of antimicrobial agents by regression analysis of light scattering data

Abstract

A novel, rapid, automated method for determining the minimum inhibitory concentrations of antimicrobial drugs within ranges acceptable for therapeutic application is described and validated in this report. By employing a simple modification of the Autobac photometer currently in use and in optically clear, modified Mueller-Hinton broth, the method utilizes forward light scattering data measured in the presence of two concentrations of the antimicrobial agent to compute the minimum inhibitory concentrations. Empirically derived regression equations which simultaneously use scattering data from two drug concentrations are employed in this computation, rather than simple breakpoint analysis in which the light scattering measured in the presence of each drug concentration is referred to a threshold level. The minimum inhibitory concentrations obtained with this new method were highly reproducible and, as shown by side by side comparisons were in excellent agreement with the minimum inhibitory cencentrations obtained with the International Collaborative Study broth dilution method.