Improved Assay for Rhodanese in Thiobacillus spp-Reference-Cited by-同舟云学术

Improved Assay for Rhodanese in Thiobacillus spp

Published:1988-11 Issue:11 Volume:54 Page:2866-2867
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Singleton David R.¹,Smith David W.¹

Affiliation:

1. School of Life and Health Sciences, University of Delaware, Newark, Delaware 19716

Abstract

Rhodanese (thiosulfate:cyanide sulfurtransferase; EC 2.8.1.1) catalyzes the conversion of thiosulfate and cyanide to thiocyanate and sulfite. Conventional rhodanese assays colorimetrically measure the formation of one or the other of the products. These assays suffer from the fact that there is significant nonbiological formation of these products in addition to the enzymatically catalyzed reaction. In the present report, we describe a modified procedure for assaying rhodanese in which a separate boiled control was prepared for each assay trial. The boiled control corrected for the nonbiological contributions to product formation.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.54.11.2866-2867.1988

Reference18 articles.

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5. Davis R. E. 1968. Mechanisms of sulfur reactions p. 85-135. In G. Nickless (ed.) Inorganic sulphur chemistry. Elsevier Science Publishing Inc. New York.

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