Affiliation:
1. Lehrstuhl für Pflanzenphysiologie, Ruhr-Universität Bochum, D-4630 Bochum, Federal Republic of Germany
Abstract
Arthrobacter
sp. strain GLP-1, grown on glucose as a carbon source, utilizes the herbicide glyphosate [
N
-(phosphonomethyl)glycine] as its sole source of phosphorus as well as its sole source of nitrogen. The mutant strain GLP-1/Nit-1 utilizes glyphosate as its sole source of nitrogen as well. In strain GLP-1, P
i
was a potent competitive inhibitor of glyphosate uptake (
K
i
, 24 μM), while the affinity of P
i
for the uptake system of strain GLP-1/Nit-1 was reduced by 2 orders of magnitude (
K
i
, 2.3 mM). It is concluded that the inability of strain GLP-1 to utilize glyphosate as a source of nitrogen is due to the stringent control of glyphosate uptake by excess phosphate released during the degradation of the herbicide.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference17 articles.
1. Glyphosate-degrading microorganisms from industrial activated sludge;Balthazor T. M.;Appl. Environ. Microbiol.,1986
2. Phosphonates as analogues of biological phosphates;Blackburn G. M.;Chem. Ind.,1981
3. Carlton B. C. and B. J. Brown. 1981. Gene mutation p. 222-229. In P. Gerhardt R. G. E. Murray R. N. Costilow E. W. Nester W. A. Wood N. R. Krieg and G. B. Phillips (ed.) Manual of methods for general bacteriology. American Society of Microbiology Washington D.C.
4. Phosphonate utilization by bacteria;Cook A. M.;J. Bacteriol.,1978
5. Drake J. L. Jr. and T. A. Calamari Jr. 1983. Industrial uses of phosphonates p. 171-194. In R. L. Hilderbrand (ed.) The role of phosphonates in living systems. CRC Press Boca Raton Fla.
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