Affiliation:
1. Department of Environmental Chemistry and Engineering, Faculty of Engineering, Oita University, Oita 870-11, Japan
Abstract
A bacterial strain that produces
d
-aminoacylase was isolated from soil and identified as
Alcaligenes denitrificans
subsp.
xylosoxydans
MI-4.
l
-Aminoacylase activity in this strain was only 1 to 2% of
d
-aminoacylase activity.
d
-Aminoacylase was inducibly produced.
N
-Acetyl-
dl
-leucine was the best inducer, and the
d
-isomer had the ability to induce the enzyme. Enzymatic resolution of
N
-acetyl-
dl
-methionine with the crude enzyme was carried out, and the
d
/
l
ratio in the resolved methionine was approximately 100/7, suggesting that resolution with crude enzymes may become possible by removing small amounts of the contaminated
l
-form with
l
-amino acid oxidase.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference17 articles.
1. Barrett G. C. (ed.). 1985. Chemistry and biochemistry of the amino acids p. 338-353. Chapman & Hall Ltd. London.
2. Chibata I. T. Tosa T. Sato T. Mori and Y. Matsuo. 1972. Preparation and industrial application of immobilized aminoacylase p. 383-389. In G. Terui (ed.) Proceedings of the IVth International Fermentation Symposium: Fermentation Technology Today. Society of Fermentation Technology Kyoto Japan.
3. Cowan S. T. 1974. Characters of gram-negative bacteria p. 77-122. In Manual for the identification of medical bacteria 2nd ed. Cambridge University Press Cambridge.
4. Holding A. J. and T. M. Shewan. 1974. Genus Alcaligenes p. 273-275. In R. E. Buchanan and N. E. Gibbons (ed.) Bergey's manual of determinative bacteriology. The Williams & Wilkins Co. Baltimore.
5. Studies on acylase activity and microorganisms. XXVI. Purification and properties of D-acylase (N-acyl-D-amino acid amidohydrolase) from AAA 6029 (Pseudomonas sp.);Kameda Y.;Chem. Pharm. Bull.,1978
Cited by
44 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献