Affiliation:
1. College of Plant Protection, State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing Agricultural University, Nanjing, China
Abstract
ABSTRACT
Xanthomonas oryzae
pv. oryzae, which causes rice bacterial leaf blight, and
Xanthomonas oryzae
pv. oryzicola, which causes rice bacterial leaf streak, are important plant-pathogenic bacteria. A member of the adaptor protein family, ankyrin protein, has been investigated largely in humans but rarely in plant-pathogenic bacteria. In this study, a novel ankyrin-like protein, AnkB, was identified in
X. oryzae
pv. oryzae and
X. oryzae
pv. oryzicola. The expression of
ankB
was significantly upregulated when these bacteria were treated with phenazine-1-carboxylic acid (PCA).
ankB
is located 58 bp downstream of the gene
catB
(which encodes a catalase) in both bacteria, and the gene expression of
catB
and catalase activity were reduced following
ankB
deletion in
X. oryzae
pv. oryzae and
X. oryzae
pv. oryzicola. Furthermore, we demonstrated that AnkB directly interacts with CatB by glutathione
S
-transferase (GST) pulldown assays. Deletion of
ankB
increased the sensitivity of
X. oryzae
pv. oryzae and
X. oryzae
pv. oryzicola to H
2
O
2
and PCA, decreased bacterial biofilm formation, swimming ability, and exopolysaccharide (EPS) production, and also reduced virulence on rice. Together our results indicate that the ankyrin-like protein AnkB has important and conserved roles in antioxidant systems and pathogenicity in
X. oryzae
pv. oryzae and
X. oryzae
pv. oryzicola.
IMPORTANCE
This study demonstrates that the ankyrin protein AnkB directly interacts with catalase CatB in
Xanthomonas oryzae
pv. oryzae and
Xanthomonas oryzae
pv. oryzicola. Ankyrin protein AnkB can affect the gene expression of
catB
, catalase activity, and sensitivity to H
2
O
2
. In
Xanthomonas
spp., the locations of genes
ankB
and
catB
and the amino acid sequence of AnkB are highly conserved. It is suggested that in prokaryotes, AnkB plays a conserved role in the defense against oxidative stress.
Funder
Special Fund for Agro-scientific Research in the Public Interest
Innovation Team Program for Jiangsu Universities
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
9 articles.
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