Diversification of Lupine
Bradyrhizobium
Strains: Evidence from Nodulation Gene Trees
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Published:2007-05-15
Issue:10
Volume:73
Page:3254-3264
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ISSN:0099-2240
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Container-title:Applied and Environmental Microbiology
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language:en
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Short-container-title:Appl Environ Microbiol
Author:
Stępkowski Tomasz1, Hughes Colin E.2, Law Ian J.3, Markiewicz Łukasz1, Gurda Dorota1, Chlebicka Agnieszka1, Moulin Lionel4
Affiliation:
1. Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61 704 Poznań, Poland 2. Department of Plant Sciences, University of Oxford, South Parks Rd., Oxford OX1 3RB, United Kingdom 3. Plant Protection Research Institute, Private Bag X134, Queenswood 0121, South Africa 4. Laboratoire des Symbioses Tropicales et Méditerranéennes, IRD-CIRAD-INRA-UMII, 34398 Montpellier Cedex 5, France
Abstract
ABSTRACT
Bradyrhizobium
strains isolated in Europe from Genisteae and serradella legumes form a distinct lineage, designated clade II, on nodulation gene trees. Clade II bradyrhizobia appear to prevail also in the soils of Western Australia and South Africa following probably accidental introduction with seeds of their lupine and serradella hosts. Given this potential for dispersal, we investigated
Bradyrhizobium
isolates originating from a range of native New World lupines, based on phylogenetic analyses of nodulation (
nodA
,
nodZ
,
noeI
) and housekeeping (
atpD
,
dnaK
,
glnII
,
recA
) genes. The housekeeping gene trees revealed considerable diversity among lupine bradyrhizobia, with most isolates placed in the
Bradyrhizobium japonicum
lineage, while some European strains were closely related to
Bradyrhizobium canariense
. The
nodA
gene tree resolved seven strongly supported groups (clades I to VII) that correlated with strain geographical origins and to some extent with major
Lupinus
clades. All European strains were placed in clade II, whereas only a minority of New World strains was placed in this clade. This work, as well as our previous studies, suggests that clade II diversified predominately in the Old World, possibly in the Mediterranean. Most New World isolates formed subclade III.2, nested in a large “pantropical” clade III, which appears to be New World in origin, although it also includes strains originating from nonlupine legumes. Trees generated using
nodZ
and
noeI
gene sequences accorded well with the
nodA
tree, but evidence is presented that the
noeI
gene may not be required for nodulation of lupine and that loss of this gene is occurring.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference52 articles.
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