Whole-Genome Mapping: a New Paradigm in Strain-Typing Technology-Reference-Cited by-同舟云学术

Whole-Genome Mapping: a New Paradigm in Strain-Typing Technology

Published:2013-04 Issue:4 Volume:51 Page:1066-1070
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Miller J. Michael¹

Affiliation:

1. Microbiology Technical Services, LLC, Dunwoody, Georgia, USA

Abstract

ABSTRACT Strain-typing technology in support of outbreak identification and resolution has evolved from phenotypic analysis, such as serology and biotypes, to much-more-robust molecular genetic approaches, such as pulsed-field gel electrophoresis (PFGE) and whole-genome sequencing. Whole-genome mapping (WGM) has been recently applied to subtyping analysis, and it bridges the gap between PFGE (∼20 bands sorted by size) and whole-genome sequencing. WGM utilizes restriction site analysis but arranges 200 to 500 bands in the order they appear on the chromosome. WGM is able to quickly and cost-effectively generate high-resolution, ordered whole-genome maps of bacteria.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00093-13

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