Quality control criteria for testing the susceptibility of anaerobic bacteria to meropenem

Author:

Zabransky R J1,Bayola-Mueller L1,Jenkins S G1,Pitkin D R1,Schreckenberger P C1,Celig D M1,Spiegel C A1

Affiliation:

1. Department of Pathology and Laboratory Medicine, University of Wisconsin Medical School, Sinai Samaritan Medical Center, Milwaukee 53201.

Abstract

Reference values for quality control of in vitro susceptibility tests with meropenem against anaerobic bacteria were determined in a multilaboratory study by the approved National Committee for Clinical Laboratory Standards agar dilution method for the four quality control strains. The study protocol also included the evaluation of microdilution testing, medium additives, and multiple lots of media. The recommended MIC control ranges for three of the control organisms are as follows: Bacteroides fragilis ATCC 25285, 0.06 to 0.125 micrograms/ml; Bacteroides thetaiotaomicron ATCC 29741, 0.125 to 0.5 micrograms/ml; and Eubacterium lentum ATCC 43055, 0.125 to 0.5 micrograms/ml. The modal MIC for Clostridium perfringens ATCC 13124 was at or below the lowest concentration of meropenem tested, and no values are recommended.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference7 articles.

1. Quality control limits for the standard anaerobic reference agar dilution susceptibility test procedure for the National Committee for Clinical Laboratory Standards;Barry A. L.;J. Clin. Microbiol.,1989

2. Eubacterium lentum ATCC 43055, a new Referencestrain for quality control of anaerobic susceptibility tests;Barry A. L.;J. Clin. Microbiol.,1990

3. In vitro antibacterial activity of SM-7338, a carbapenem antibiotic with stability to dehydropeptidase I;Edwards J. R.;Antimicrob. Agents Chemother.,1989

4. National Committee for Clinical Laboratory Standards. 1987. Development of in vitro susceptibility criteria and quality control parameters; proposed guideline. M26-P. National Committee for Clinical Laboratory Standards Villanova Pa.

5. National Committee for Clinical Laboratory Standards. 1989. Methods for antimicrobial susceptibility testing of anaerobic bacteria 2nd ed. M11-T2. National Committee for Clinical Laboratory Standards Villanova Pa.

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