Affiliation:
1. Department of Biological Chemistry, John Innes Centre, Norwich, United Kingdom
Abstract
The mechanism whereby members of the order
Picornavirales
specifically package their genomic RNAs is poorly understood. Research with monopartite members of the order, such as poliovirus, indicated that packaging is linked to replication, although the presence of “packaging signals” along the length of the viral RNA has also been suggested. Thanks to the bipartite nature of the CPMV genome, which allows the manipulation of RNA-1 without modifying RNA-2, we show here that this specificity is due to a functional link between the two processes of viral replication and encapsidation. This has important implications for our understanding of the fundamental molecular biology of
Picornavirales
and opens the door to novel research and therapeutic applications in the field of custom RNA packaging and delivery technologies.
Funder
RCUK | Biotechnology and Biological Sciences Research Council
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Cited by
15 articles.
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