Performance of the 47-Kilodalton Membrane Protein versus DNA Polymerase I Genes for Detection of Treponema pallidum by PCR in Ulcers-Reference-Cited by-同舟云学术

Performance of the 47-Kilodalton Membrane Protein versus DNA Polymerase I Genes for Detection of Treponema pallidum by PCR in Ulcers

Published:2014-12-17 Issue:3 Volume:53 Page:976-980
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J. Clin. Microbiol.

Author:

Gayet-Ageron Angèle,Laurent Frédéric,Schrenzel Jacques,Charton Béatrice,Jimenez-Getaz Gisela,Tangomo Manuela,Ferry Tristan,Sednaoui Patrice,Lautenschlager Stephan,Toutous-Trellu Laurence,Martinez de Tejada Begoña,Cavassini Matthias,Emonet Stéphane,Perneger Thomas,Salord Hélène

Abstract

Treponema pallidumPCR (Tp-PCR) is a direct diagnostic method for primary and secondary syphilis, but there is no recommendation regarding the best choice of target gene. In this study, we sequentially tested 272 specimens from patients with sexually transmitted ulcers usingTp-PCR targeting thetpp47and thenpolAgenes. The two methods showed similar accuracies and an almost-perfect agreement.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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