Affiliation:
1. Laboratoire de Microbiologie, UMR INRA UB 1232, ENSBANA, 1 Esplanade Erasme, 21000 Dijon, France
Abstract
ABSTRACT
LuxS is responsible for the production of autoinducer 2 (AI-2), which is involved in the quorum-sensing response of
Vibrio harveyi
. AI-2 is found in several other gram-negative and gram-positive bacteria and is therefore considered a good candidate for an interspecies communication signal molecule. In order to determine if this system is functional in the gastrointestinal pathogen
Listeria monocytogenes
EGD-e, an AI-2 bioassay was performed with culture supernatants. The results indicated that this bacterium produces AI-2 like molecules. A potential ortholog of
V. harveyi luxS
, lmo1288, was found by performing sequence similarity searches and complementation experiments with
Escherichia coli
DH5α, a
luxS
null strain. lmo1288 was found to be a functional
luxS
ortholog involved in AI-2 synthesis. Indeed, interruption of lmo1288 resulted in loss of the AI-2 signal. Although no significant differences were observed between Lux1 and EGD-e with regard to planktonic growth (at 10°C, 15°C, 25°C, and 42°C), swimming motility, and phospholipase and hemolytic activity, biofilm culture experiments showed that under batch conditions between 25% and 58% more Lux1 cells than EGD-e cells were attached to the surface depending on the incubation time. During biofilm growth in continuous conditions after 48 h of culture, Lux1 biofilms were 17 times denser than EGD-e biofilms. Finally, our results showed that Lux1 accumulates more
S
-adenosyl homocysteine (SAH) and
S
-ribosyl homocysteine (SRH) in culture supernatant than the parental strain accumulates and that SRH, but not SAH or AI-2, is able to modify the number of attached cells.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
85 articles.
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