Overexpression of the 14α-Demethylase Target Gene ( CYP51 ) Mediates Fungicide Resistance in Blumeriella jaapii-Reference-Cited by-同舟云学术

Overexpression of the 14α-Demethylase Target Gene ( CYP51 ) Mediates Fungicide Resistance in Blumeriella jaapii

Published:2006-04 Issue:4 Volume:72 Page:2581-2585
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Ma Zhonghua¹,Proffer Tyre J.¹²,Jacobs Janette L.¹,Sundin George W.¹³

Affiliation:

1. Department of Plant Pathology

2. Department of Biological Science, Kent State University, Kent, Ohio

3. Center for Microbial Ecology, Michigan State University, East Lansing, Michigan

Abstract

ABSTRACT Sterol demethylation inhibitor (DMI) fungicides are widely used to control fungi pathogenic to humans and plants. Resistance to DMIs is mediated either through alterations in the structure of the target enzyme CYP51 (encoding 14α-demethylase), through increased expression of the CYP51 gene, or through increased expression of efflux pumps. We found that CYP51 expression in DMI-resistant (DMI R ) isolates of the cherry leaf spot pathogen Blumeriella jaapii was increased 5- to 12-fold compared to that in DMI-sensitive (DMI S ) isolates. Analysis of sequences upstream of CYP51 in 59 DMI R isolates revealed that various forms of a truncated non-long terminal direct repeat long interspersed nuclear element retrotransposon were present in all instances. Similar inserts upstream of CYP51 were not present in any of 22 DMI S isolates examined.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.72.4.2581-2585.2006

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