Further Verification of the Isotope Dilution Approach for Estimating the Degree of Participation of [ 3 H]thymidine in DNA Synthesis in Studies of Aquatic Bacterial Production

Abstract

The optimal concentration of [ 3 H]thymidine (i.e., the maximal degree of participation in DNA synthesis) as determined by adding increasing amounts of labeled thymidine at the same specific activity was similar to the concentration of thymidine inhibiting the de novo pathway as determined by isotope dilution plots. These experiments provide further verification of the isotope dilution approach for determining the degree of participation of [ 3 H]thymidine in DNA synthesis.