The Oligomeric State of c Rings from Cyanobacterial F-ATP Synthases Varies from 13 to 15

Abstract

ABSTRACT We isolated the c rings of F-ATP synthases from eight cyanobacterial strains belonging to four different taxonomic classes ( Chroococcales , Nostocales , Oscillatoriales , and Gloeobacteria ). These c rings showed different mobilities on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), probably reflecting their molecular masses. This supposition was validated with the previously characterized c 11 , c 14 , and c 15 rings, which migrated on SDS-PAGE in proportion to their molecular masses. Hence, the masses of the cyanobacterial c rings can conveniently be deduced from their electrophoretic mobilities and, together with the masses of the c monomers, allow the calculation of the c ring stoichiometries. The method is a simple and fast way to determine stoichiometries of SDS-stable c rings and hence a convenient means to unambiguously determine the ion-to-ATP ratio, a parameter reflecting the bioenergetic efficacy of F-ATP synthases. AFM imaging was used to prove the accuracy of the method and confirmed that the c ring of Synechococcus elongatus SAG 89.79 is a tridecameric oligomer. Despite the high conservation of the c-subunit sequences from cyanobacterial strains from various environmental groups, the stoichiometries of their c rings varied between c 13 and c 15 . This systematic study of the c-ring stoichiometries suggests that variability of c-ring sizes might represent an adaptation of the individual cyanobacterial species to their particular environmental and physiological conditions. Furthermore, the two new examples of c 15 rings underline once more that an F 1 /F o symmetry mismatch is not an obligatory feature of all F-ATP synthases.