Oligonucleotide microchips as genosensors for determinative and environmental studies in microbiology

Author:

Guschin D Y1,Mobarry B K1,Proudnikov D1,Stahl D A1,Rittmann B E1,Mirzabekov A D1

Affiliation:

1. Joint Human Genome Program, Argonne National Laboratory, Illinois 60439, USA.

Abstract

The utility of parallel hybridization of environmental nucleic acids to many oligonucleotides immobilized in a matrix of polyacrylamide gel pads on a glass slide (oligonucleotide microchip) was evaluated. Oligonucleotides complementary to small-subunit rRNA sequences of selected microbial groups, encompassing key genera of nitrifying bacteria, were shown to selectively retain labeled target nucleic acid derived from either DNA or RNA forms of the target sequences. The utility of varying the probe concentration to normalize hybridization signals and the use of multicolor detection for simultaneous quantitation of multiple probe-target populations were demonstrated.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference36 articles.

1. The oligonucleotide probe database (OPD);Alm E. W.;Appl. Environ. Microbiol.,1996

2. Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations;Amann R. I.;Appl. Environ. Microbiol.,1990

3. Phylogenetic identification and in situ detection of individual microbial cells without cultivation;Amann R. I.;Microbiol. Rev.,1995

4. Ausubel F. M. R. Brent R. E. Kingston D. D. Moore J. G. Seidman J. A. Smith and K. Struhl (ed.). 1994. Current protocols in molecular biology. Greene Publishing Associates and Wiley Interscience New York N.Y.

5. Remarkable archaeal diversity detected in a Yellowstone National Park hot spring environment;Barns S. M.;Proc. Natl. Acad. Sci. USA,1994

Cited by 273 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3