Novel Monomeric Fungal Subtilisin Inhibitor from a Plant-Pathogenic Fungus, Choanephora cucurbitarum: Isolation and Molecular Characterization

Author:

Pathiraja Duleepa1,Chun Youngeun1,Cho Junghwan1,Min Byoungnam1,Lee Saeyoung1,Park Hongjae1,Byun Juan1,Choi In-Geol1ORCID

Affiliation:

1. Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea

Abstract

Until recently, Streptomyces subtilisin inhibitors (SSI) were reported and characterized only in bacteria. We found SSI-like domains in a plant-pathogenic fungus, Choanephora cucurbitarum KUS-F28377, which contains 11 sequentially diverged SSI-like domains. None of these fungal SSI-like domains were functionally characterized before. The active form of fungal SSI-like protein is a monomer, in contrast to the homodimeric bacterial SSI. We constructed a synthetic monomer of bacterial SSI to demonstrate the modulation of its activity based on structural integrity and not reactive sites. Our results suggest the duplication and divergence of SSI-like domains of C. cucurbitarum within the genome to inhibit various cognate proteases during evolution by modulating both binding and reactivity. The molecular functional characterization of fungal SSI-like domains will be useful in understanding their biological role and future biotechnological applications.

Funder

Rural Development Administration

National Research Foundation of Korea

MOTIE | Korea Institute of Energy Technology Evaluation and Planning

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

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