Author:
Ise Y,Iida T,Sato K,Suzuki T,Shimada K,Nishioka K
Abstract
Experiments were performed to investigate the usefulness of an enzyme-linked immunosorbent assay utilizing avidin-biotin interaction as a diagnostic tool for detection of Toxoplasma antigen in blood. The lower limit of sensitivity of the assay by this method was ca. 4 ng/ml, and standard assays provided a linear plot of antigen concentration over a range up to 250 ng/ml. In rabbits inoculated subcutaneously with trophozoites of the RH strain, Toxoplasma antigen became demonstrable in the circulation 3 days after injection, before emergence of antibody in serum and development of parasitemia. Analysis of the antigen in serum from infected rabbits by high-permeation liquid gel chromatography suggested the occurrence of antigens of four different molecular weights, greater than or equal to 400,000, 220,000, 130,000, and 45,000. Of these antigens, those of molecular weights 220,000 and 130,000 showed a conspicuous elevation with time after infection.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Reference22 articles.
1. Cerebral toxoplasmosis in acquired immune deficiency syndrome;Alonso R.;Arch. Neurol.,1984
2. Antigenemia recently acquired acute toxoplasmosis;Araujo F. G.;J. Infect. Dis.,1980
3. Diagnostic information from serological tests in human toxoplasmosis. I. A comparative study of hemagglutination, complement fixation, IgG and IgM-immunofluorescence tests in 3,752 serum samples;Camargo M. E.;Rev. Inst. Med. Trop. Sao Paulo,1976
4. Value of Iymphnode biopsy in the diagnosis of acute acquired toxoplasmosis;Dorfman R. F.;N. Engl. J. Med.,1973
5. The use of avidin-biotin interaction in immunoenzymatic techniques;Guesdon J.;J. Histochem. Cytochem.,1979
Cited by
19 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献