Transformation of the dermatophyte Trichophyton mentagrophytes to hygromycin B resistance-Reference-Cited by-同舟云学术

Transformation of the dermatophyte Trichophyton mentagrophytes to hygromycin B resistance

Published:1989-09 Issue:9 Volume:57 Page:2923-2925
ISSN:0019-9567
Container-title:Infection and Immunity
language:en
Short-container-title:Infect Immun

Author:

Gonzalez R¹,Ferrer S¹,Buesa J¹,Ramon D¹

Affiliation:

1. Departament de Microbiologia, Facultat de Biologia, Universitat de Valéncia, Spain.

Abstract

A transformation system for the ringworm-producing dermatophyte Trichophyton mentagrophytes has been developed. The system employs the plasmid pHIS, which contains a bacterial hygromycin B phosphotransferase gene linked to Cochliobolus heterostrophus regulatory sequences (B. G. Turgeon, R. C. Garber, and O. C. Yoder, Mol. Cell. Biol. 7:3297-3305, 1987). This plasmid confers hygromycin B resistance to T. mentagrophytes. The DNA was stably integrated into the fungal genome, and the number and sites of integrations varied among transformants. Transformant clones were capable of infecting guinea pigs. This system opens the way for the molecular genetic analysis of the interaction of T. mentagrophytes with epithelial animal tissues.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Link

https://journals.asm.org/doi/pdf/10.1128/iai.57.9.2923-2925.1989

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