Affiliation:
1. Department of Biological Sciences, The University at Buffalo, Buffalo, New York 14260
2. Department of Biology, Carleton University, Ottawa, Ontario, Canada K1S 5B6
Abstract
ABSTRACT
Using mutational and proteomic approaches, we have demonstrated the importance of the glycosylphosphatidylinositol (GPI) anchor pathway for cell wall synthesis and integrity and for the overall morphology of the filamentous fungus
Neurospora crassa
. Mutants affected in the
gpig-1
,
gpip-1
,
gpip-2
,
gpip-3
, and
gpit-1
genes, which encode components of the
N. crassa
GPI anchor biosynthetic pathway, have been characterized. GPI anchor mutants exhibit colonial morphologies, significantly reduced rates of growth, altered hyphal growth patterns, considerable cellular lysis, and an abnormal “cell-within-a-cell” phenotype. The mutants are deficient in the production of GPI-anchored proteins, verifying the requirement of each altered gene for the process of GPI-anchoring. The mutant cell walls are abnormally weak, contain reduced amounts of protein, and have an altered carbohydrate composition. The mutant cell walls lack a number of GPI-anchored proteins, putatively involved in cell wall biogenesis and remodeling. From these studies, we conclude that the GPI anchor pathway is critical for proper cell wall structure and function in
N. crassa
.
Publisher
American Society for Microbiology
Subject
Molecular Biology,General Medicine,Microbiology
Cited by
69 articles.
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