Alternate Polypurine Tracts Affect Rous Sarcoma Virus Integration In Vivo-Reference-Cited by-同舟云学术

Alternate Polypurine Tracts Affect Rous Sarcoma Virus Integration In Vivo

Published:2006-10-15 Issue:20 Volume:80 Page:10281-10284
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Oh Jangsuk¹,Chang Kevin W.¹,Alvord W. Gregory²,Hughes Stephen H.¹

Affiliation:

1. HIV Drug Resistance Program

2. Data Management Services, National Cancer Institute at Frederick, Frederick, Maryland 21702-1201

Abstract

ABSTRACT When the endogenous polypurine tract (PPT) of the Rous sarcoma virus (RSV)-derived vector RSVP(A)Z was replaced with alternate retroviral PPTs, the fraction of unintegrated viral DNA with the normal consensus ends significantly decreased and the retention of part of the PPT significantly increased. If the terminus of the U3 long terminal repeat (LTR) is aberrant, RSV integrase can correctly process and integrate the normal U5 LTR into the host genome. However, the canonical CA is not involved in joining the aberrant U3 LTR to the host DNA, generating either large duplications or deletions of the host sequences instead of the normal 5- or 6-bp duplication.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.00361-06

Reference12 articles.

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