A cytoplasmic protein, NfrC, is required for bacteriophage N4 adsorption-Reference-Cited by-同舟云学术

A cytoplasmic protein, NfrC, is required for bacteriophage N4 adsorption

Published:1993-11 Issue:21 Volume:175 Page:7074-7080
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Kiino D R¹,Licudine R¹,Wilt K¹,Yang D H¹,Rothman-Denes L B¹

Affiliation:

1. Department of Molecular Genetics and Cell Biology, University of Chicago, Illinois 60637.

Abstract

At least four genes are required for irreversible adsorption of bacteriophage N4. nfrA and nfrB have been characterized previously and encode an outer membrane protein and inner membrane protein, respectively. The nfrC gene product is characterized in detail in this study. We have mapped the nfrD locus to min 52 on the Escherichia coli linkage map. Maxicell analysis of nfrC and a null allele (nfrC2) cloned into a high-copy-number plasmid shows its gene product to be 42 kDa in size. We determined the nfrC nucleotide sequence which predicts a gene product of 42 kDa. Western blots (immunoblots) of Escherichia coli proteins after cellular fractionation show NfrC to be a cytoplasmic protein which is required for irreversible bacteriophage N4 adsorption, an event occurring at the cell surface.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.175.21.7074-7080.1993

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