Molecular Characterization of a Novel Plasmid-Encoded Cefotaximase (CTX-M-12) Found in Clinical Klebsiella pneumoniae Isolates from Kenya-Reference-Cited by-同舟云学术

Molecular Characterization of a Novel Plasmid-Encoded Cefotaximase (CTX-M-12) Found in Clinical Klebsiella pneumoniae Isolates from Kenya

Published:2001-07 Issue:7 Volume:45 Page:2141-2143
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Kariuki S.¹²,Corkill J. E.²,Revathi G.³,Musoke R.³,Hart C. A.²

Affiliation:

1. Centre for Microbiology Research, Kenya Medical Research Institute,1 and

2. Department of Medical Microbiology and Genito-Urinary Medicine, University of Liverpool, Liverpool L69 3GA, United Kingdom2

3. Department of Medical Microbiology, Kenyatta National Hospital,3 Nairobi, Kenya, and

Abstract

ABSTRACT Nine Klebsiella pneumoniae isolates, six from blood and three from cerebrospinal fluid of newborn babies at Kenyatta National Hospital, Nairobi, Kenya, were analyzed for the mechanism of cephalosporin resistance. By using pulsed-field gel electrophoresis of Xba I-digested chromosomal DNA, all the nine isolates were found to be clonal. PCR and direct sequencing revealed a novel extended-spectrum β-lactamase, which we designated CTX-M-12. It has a more potent hydrolytic activity against cefotaxime than against ceftazidime and a pI of 9.0 and is encoded on a large self-transferable ca. 160-kbp plasmid.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.45.7.2141-2143.2001

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