Selective Amplification of tyv (rfbE), prt (rfbS), viaB , and fliC Genes by Multiplex PCR for Identification of Salmonella enterica Serovars Typhi and Paratyphi A-Reference-Cited by-同舟云学术

Selective Amplification of tyv (rfbE), prt (rfbS), viaB , and fliC Genes by Multiplex PCR for Identification of Salmonella enterica Serovars Typhi and Paratyphi A

Published:2002-02 Issue:2 Volume:40 Page:633-636
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Hirose Kenji¹,Itoh Ken-Ichiro²,Nakajima Hiroshi³,Kurazono Takayuki⁴,Yamaguchi Masanori⁴,Moriya Kazuo⁵,Ezaki Takayuki⁶,Kawamura Yoshiaki⁶,Tamura Kazumichi¹,Watanabe Haruo¹

Affiliation:

1. Department of Bacteriology, National Institute of Infectious Diseases, Tokyo 162-8640

2. Department of Microbiology, National Institute of Public Health, Tokyo 108-8638

3. Department of Microbiology, Okayama Prefectural Institute for Environmental Science and Public Health, Okayama 701-0298

4. Saitama Institute for Public Health, Saitama 338-0842

5. Saga Prefectural Institute of Public Health, Saga 849-0925

6. Department of Microbiology, Gifu University, School of Medicine, Gifu 500-8750, Japan

Abstract

ABSTRACT The PCR primers for O, H, and Vi antigen genes, tyv (rfbE), prt ( rfbS ), fliC-d, fliC-a , and viaB , were designed and used for the rapid identification of Salmonella enterica serovars Typhi and Paratyphi A with multiplex PCR. The results showed that all the clinical isolates examined of Salmonella serovars Typhi and Paratyphi A were accurately identified by this assay.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.40.02.633-636.2002

Reference9 articles.

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3. Development of nested PCR based on the ViaB sequence to detect Salmonella typhi

4. Antibiotic Susceptibilities of Salmonella enterica Serovar Typhi and S. enterica Serovar Paratyphi A Isolated from Patients in Japan

5. Kauffman, F. 1936. Über die diphasische Natur der Typhusbacillen. Z. Hyg. 119:103-118.

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