Biological activity of a lipopolysaccharide extracted from Treponema hyodysenteriae

Abstract

A lipopolysaccharide (LPS) was obtained from pathogenic Treponema hyodysenteriae by hot phenol-water extraction. Various effects of the LPS on host cells were examined in vitro. Toxicity for mouse peritoneal macrophages was observed after 10 h of incubation at concentrations as low as 15 micrograms of the LPS per ml. Marked enhancement of both complement (C3) and immunoglobulin G-Fc receptor-mediated internalization was noted in macrophages obtained from mice injected 6 days previously with 75 micrograms of the material. Incorporation of [3H]thymidine into murine splenocytes was elevated approximately fourfold when splenocytes were treated with 5 to 10 micrograms of LPS per ml. Incubation of the LPS with normal porcine serum resulted in the generation of a factor(s) that stimulated the migration of porcine leukocytes. Generation of the chemotactic activity was inhibited by heating the serum at 56 degrees C for 30 min before treatment with LPS. The results suggest that T. hyodysenteriae contains an LPS that is biologically active.