Detection of antibodies to caprine arthritis-encephalitis virus by protein G enzyme-linked immunosorbent assay and immunoblotting

Author:

Zanoni R1,Krieg A1,Peterhans E1

Affiliation:

1. Institute of Veterinary Virology, University of Bern, Switzerland.

Abstract

Sera from goats suffering from caprine arthritis-encephalitis contained antibodies to virus proteins of 15, 17, 28, 40, and 130 kilodaltons in immunoblots of maedi-visna virus. We propose to use immunoblotting as a validation test for enzyme-linked immunosorbent assay and demonstrate that the specificity of indirect enzyme-linked immunosorbent assay can be improved by replacing second antibody by a protein G-avidin-biotin conjugate.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference16 articles.

1. Identification of caprine arthritis-encephalitis retrovirus proteins in immunodiffusion precipitin lines;Adams D. S.;J. Gen. Virol.,1985

2. Protein G: a powerful tool for binding and detection of monoclonal and polyclonal antibodies;Akerstrom B.;J. Immunol.,1985

3. Development of an enzyme-linked immunosorbent assay for caprine arthritis-encephalitis virus;Archambault D.;J. Clin. Microbiol.,1988

4. Preparation and evaluation of antigens used in serological tests for caprine syncytial retrovirus antibody in sheep and goat sera;Coackley W.;Vet. Microbiol.,1984

5. Dawson M. P. Biront and D. J. Houwers. 1985. A comparison of an indirect enzyme-linked immunosorbent assay an indirect immunofluorescence test and an agar gel diffusion test for the detection of antibodies to maedi/visna virus in ovine sera p. 93-104. In J. M. Sharp and R. Hoff-Jorgensen (ed.) Slow viruses in sheep goats and cattle: proceedings of two workshops one held in Reykjavik (Iceland) on 13 and 14 July 1982 the other in Edinburgh (Scotland) on 13 and 14 September 1983. Commission of the European Communities Luxembourg.

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