Affiliation:
1. Department of Microbiology and Cell Science and Department of Immunology and Medical Microbiology, 2 University of Florida, Gainesville, Florida 32611
Abstract
The potential utility of
Zymomonas mobilis
as an organism for the commercial production of ethanol would be greatly enhanced by the addition of foreign genes which expand its range of fermentable substrates. We tested various plasmids and mobilizing factors for their ability to act as vectors and introduce foreign genes into
Z. mobilis
CP4. Plasmid pGC91.14, a derivative of RP1, was found to be transferred from
Escherichia coli
to
Z. mobilis
at a higher frequency than previously reported for any other plasmids. Both tetracycline resistance and the lactose operon from this plasmid were expressed in
Z. mobilis
CP4. Plasmid pGC91.14 was stably maintained in
Z. mobilis
at 30°C but rapidly lost at 37°C.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
63 articles.
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