Differentiation of Mycobacterium tuberculosis Complex and Nontuberculous Mycobacterial Liquid Cultures by Using Peptide Nucleic Acid-Fluorescence In Situ Hybridization Probes-Reference-Cited by-同舟云学术

Differentiation of Mycobacterium tuberculosis Complex and Nontuberculous Mycobacterial Liquid Cultures by Using Peptide Nucleic Acid-Fluorescence In Situ Hybridization Probes

Published:2000-01 Issue:1 Volume:38 Page:444-447
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Drobniewski F. A.¹,More P. G.¹,Harris G. S.¹

Affiliation:

1. PHLS Mycobacterium Reference Unit, Department of Microbiology, Dulwich Public Health Laboratory, Guy's King's and St Thomas' Medical School, Dulwich Hospital, London SE22 8QF, United Kingdom

Abstract

ABSTRACT A blinded comparison of peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) with routine identification methods was performed on 74 consecutively positive mycobacterial liquid cultures. All Mycobacterium tuberculosis cultures (48 of 48) and 22 of 27 (81.5%) nontuberculous cultures were correctly identified (including one mixed culture). Five isolates yielded no reaction with either probe and were identified as Mycobacterium xenopi , Mycobacterium fortuitum , or Mycobacterium flavescens .

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.38.1.444-447.2000

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