Affiliation:
1. Eijkman-Winkler Institute, Utrecht University, 3584 CX Utrecht, The Netherlands,1and
2. Institute for Medical Microbiology and Virology, University Hospital Düsseldorf, D-40225 Düsseldorf, Germany2
Abstract
ABSTRACT
Nosocomial isolates of
Pseudomonas aeruginosa
and
Acinetobacter
spp. exhibit high rates of resistance to antibiotics and are often multidrug resistant. In a previous study (D. Milatovic, A. Fluit, S. Brisse, J. Verhoef, and F. J. Schmitz, Antimicrob. Agents Chemother. 44:1102–1107, 2000), isolates of these species that were resistant to sitafloxacin, a new advanced-generation fluoroquinolone with a high potency and a broad spectrum of antimicrobial activity, were found in high proportion in 23 European hospitals. Here, we investigate the clonal diversity of the 155
P. aeruginosa
and 145
Acinetobacter
spp. sitafloxacin-resistant isolates from that study by automated ribotyping. Numerous ribogroups (sets of isolates with indistinguishable ribotypes) were found among isolates of
P. aeruginosa
(
n
= 34) and
Acinetobacter
spp. (
n
= 16), but the majority of the isolates belonged to a limited number of major ribogroups. Sitafloxacin-resistant isolates (MICs > 2 mg/liter, used as a provisional breakpoint) showed increased concomitant resistance to piperacillin, piperacillin-tazobactam, ceftriaxone, ceftazidime, amikacin, gentamicin, and imipenem. The major ribogroups were repeatedly found in isolates from several European hospitals; these isolates showed higher levels of resistance to gentamicin and imipenem, and some of them appeared to correspond to previously described multidrug-resistant international clones of
P. aeruginosa
(serotype O:12) and
Acinetobacter baumannii
(clones I and II). Automated ribotyping, when used in combination with more discriminatory typing methods, may be a convenient library typing system for monitoring future epidemiological dynamics of geographically widespread multidrug-resistant bacterial clones.
Publisher
American Society for Microbiology
Cited by
57 articles.
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