Comparison between Plasma and Whole Blood Specimens for Detection of Aspergillus DNA by PCR-Reference-Cited by-同舟云学术

Comparison between Plasma and Whole Blood Specimens for Detection of Aspergillus DNA by PCR

Published:2000-10 Issue:10 Volume:38 Page:3830-3833
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Loeffler Juergen¹,Hebart Holger¹,Brauchle Ulrike¹,Schumacher Ulrike²,Einsele Hermann¹

Affiliation:

1. Medizinische Klinik, Abteilung II,1 and

2. Hygieneinstitut,2Eberhard-Karls-Universität Tübingen, 72076 Tübingen, Germany

Abstract

ABSTRACT Ninety-six plasma and whole blood specimens from nine selected patients were analyzed for the presence of Aspergillus DNA. Nineteen specimens from three patients with proven aspergillosis were PCR positive in both materials, whereas an additional 22 were PCR positive in whole blood only. All 36 samples from six patients without signs of aspergillosis were negative in both assays. We conclude that although plasma and whole blood spiked with Aspergillus conidia showed an identical lower detection limit (10 CFU), the sensitivity of plasma PCR was lower than that of PCR performed on whole blood samples.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.38.10.3830-3833.2000

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