Rapid and Sensitive Plate Method for Detection of Aspergillus fumigatus

Author:

Bauters T. G. M.1,Nelis H. J.1

Affiliation:

1. Laboratory of Pharmaceutical Microbiology, Department of Pharmaceutical Analysis, Ghent University, B-9000 Ghent, Belgium

Abstract

ABSTRACT The routine identification of Aspergillus fumigatus in clinical samples involves, apart from direct examination, the isolation of the organism on a plate followed by its microscopic characterization. This approach lacks sensitivity, specificity, and speed. A new procedure has been developed combining microcolony formation on a nylon membrane filter at 45°C with the detection of a specific 4-methylumbelliferyl-α- l -arabinopyranoside cleaving enzyme activity in digitonin permeabilized cells. The test takes approximately 14 h and has an efficiency of 98.2% and false-positive and -negative rates of 0 and 3.1%, respectively. When applied to 188 clinical samples taken from patients with proven or nonproven presence of Aspergillus species, a good agreement with the conventional plate-microscopy method was obtained.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference17 articles.

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