Affiliation:
1. Department of Microbiology
2. Institute for Comparative and Environmental Toxicology, Cornell University, Ithaca, New York 14853
Abstract
ABSTRACT
Pseudomonas putida
DTB grew aerobically with
N,N
-diethyl-
m
-toluamide (DEET) as a sole carbon source, initially breaking it down into 3-methylbenzoate and diethylamine. The former was further metabolized via 3-methylcatechol and
meta
ring cleavage. A gene from DTB,
dthA
, was heterologously expressed and shown to encode the ability to hydrolyze DEET into 3-methylbenzoate and diethylamine.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
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