Affiliation:
1. Department of Microbiology
2. Institute for Comparative and Environmental Toxicology, Cornell University, Ithaca, New York 14853
Abstract
ABSTRACT
Pseudomonas putida
DTB grew aerobically with
N,N
-diethyl-
m
-toluamide (DEET) as a sole carbon source, initially breaking it down into 3-methylbenzoate and diethylamine. The former was further metabolized via 3-methylcatechol and
meta
ring cleavage. A gene from DTB,
dthA
, was heterologously expressed and shown to encode the ability to hydrolyze DEET into 3-methylbenzoate and diethylamine.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference19 articles.
1. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
2. Barends, T. R., J. J. Polderman-Tijmes, P. A. Jekel, C. M. Hensgens, E. J. de Vries, D. B. Janssen, and B. W. Dijkstra. 2003. The sequence and crystal structure of the alpha-amino acid ester hydrolase from Xanthomonas citri define a new family of beta-lactam antibiotic acylases. J. Biol. Chem.278:23076-23084.
3. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
4. Focht, D. D. 1994. Microbial procedures for biodegradation research, p. 407-426. In R. W. Weaver, J. S. Angle, and P. S. Bottomley (ed.), Methods of soil analysis, part 2. Microbiological and biochemical properties. Soil Science Society of America, Madison, WI.
5. Kolpin, D. W., E. T. Furlong, M. T. Meyer, E. M. Thurman, S. D. Zaugg, L. B. Barber, and H. T. Buxton. 2002. Pharmaceuticals, hormones, and other organic wastewater contaminants in U.S. streams, 1999-2000: a national reconnaissance. Environ. Sci. Technol.36:1202-1211.
Cited by
39 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献