Affiliation:
1. King's College London Dental Institute, Infection Research Group, London SE1 9RT, United Kingdom
Abstract
ABSTRACT
Members of the phylum “
Synergistetes
” have frequently been detected in the human oral cavity at sites of dental disease, but they have rarely been detected in studies of oral health. Only two oral “
Synergistetes
” taxa are cultivable. The aims of this study were to investigate the diversity of “
Synergistetes
” in the oral cavity, to establish whether “
Synergistetes
” taxa are more strongly associated with periodontitis than with oral health, and to visualize unculturable “
Synergistetes
” in situ. Sixty samples (saliva, dental plaque, and mucosal swabs) were collected from five subjects with periodontitis and five periodontally healthy controls. Using phylum-specific 16S rRNA gene primers, “
Synergistetes
” were identified by PCR, cloning, and sequencing of 48 clones per PCR-positive sample. Subgingival plaque samples were labeled with probes targeting rRNA of unculturable oral “
Synergistetes
” using fluorescent in situ hybridization (FISH). Analysis of 1,664 clones revealed 12 “
Synergistetes
” operational taxonomic units (OTUs) at the 99% sequence identity level, 5 of which were novel. “
Synergistetes
” OTU 4.2 was found in significantly more subjects with periodontitis than controls (
P
= 0.048) and was more abundant in subgingival plaque at diseased sites than at healthy sites in subjects with periodontitis (
P
= 0.019) or controls (
P
= 0.019). FISH analysis revealed that unculturable oral “
Synergistetes
” cells were large curved bacilli. The human oral cavity harbors a diverse population of “
Synergistetes.
” “
Synergistetes
” OTU 4.2 is associated with periodontitis and may have a pathogenic role.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
71 articles.
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