Abstract
In Klebsiella pneumoniae, Mo accumulation appeared to be coregulated with nitrogenase synthesis. O2 and NH+4, which repressed nitrogenase synthesis, also prevented Mo accumulation. In Azotobacter vinelandii, Mo accumulation did not appear to be regulated Mo was accumulated to levels much higher than those seen in K. pneumoniae even when nitrogenase synthesis was repressed. Accumulated Mo was bound mainly to a Mo storage protein, and it could act as a supply for the Mo needed in component I synthesis when extracellular Mo had been exhausted. When A. vinelandii was grown in the presence of WO2-(4) rather than MoO2-(4), it synthesized a W-containing analog of the Mo storage protein. The Mo storage protein was purified from both NH+4 and N2-grown cells of A. vinelandii and found to be a tetramer of two pairs of different subunits binding a minimum of 15 atoms of Mo per tetramer.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference26 articles.
1. Tungsten incorporation into Azotobacter vinelandii nitrogenase;Benemann J. R.;FEBS Lett.,1973
2. Effect of molybdenum starvation and tungten on the synthesis of nitrogenase components in KkbsieUa pneumoniae;Brill W. J.;J. Bacteriol.,1974
3. Detection of nitrogenase components and other non-heme iron proteins in polyacrylamide gela Anal;Brill W. J;Biochem.,1974
4. A non-gummy chromogenic strain of Azotobacter vinelandii;Bush J. A.;Nature (London),1959
5. Determination of molybdenum and tungsten in biological materiah Anal;Cardenas J.;Biochem.,1974
Cited by
108 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献