Effects of exogenous agents on the action of Bordetella parapertussis heat-labile toxin on guinea pig skin

Author:

Endoh M1,Nagai M1,Burns D L1,Manclark C R1,Nakase Y1

Affiliation:

1. Department of Microbiology, School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan.

Abstract

Injection of sonic extracts of Bordetella parapertussis into the shaved backs of guinea pigs produced hemorrhagic necrosis, which previously has been attributed to the action of heat-labile toxin. As heat-labile toxin was purified from this crude mixture, its ability to induce hemorrhagic lesions decreased significantly. However, ischemic lesions were apparent after injection of the purified toxin. These lesions, while not hemorrhagic in nature, were marked by erythema surrounded by a region in which the ischemia was apparent. Exogenous agents were found to alter the nature of the skin lesion induced by heat-labile toxin. The lipid A portion of endotoxin in combination with heat-labile toxin caused hemorrhagic lesions surrounded by a ring of ischemia, whereas bovine serum albumin increased the area of erythema. While the nature of lesions induced by heat-labile toxin was affected by exogenous agents, the diameter of ischemia produced by the toxin was found to be independent of the presence of these agents and was linear with toxin dose. These results indicate that induction of hemorrhagic necrosis may not be a reliable indicator of heat-labile toxin activity. Instead, measurement of the ischemic lesion produced by heat-labile toxin may be a useful assay for the toxin.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Reference27 articles.

1. Some observations made during experiments on mice inoculated with H. pertussis;Andersen E. K.;Acta Pathol. Microbiol. Scand.,1952

2. L'endotoxine coqueluchense;Bordet J.;Ann. Inst. Pasteur (Paris),1909

3. Report of the task force on pertussis and pertussis immunization;Cherry J. D.;Pediatrics,1988

4. Intracellular localization of the dermonecrotic toxin of Bordetella pertussis;Coweli J. L.;Infect. Immun.,1979

5. Purification and characterization of heat-labile toxin from Bordetella bronchiseptica;Endoh M.;Microbiol. Immunol.,1986

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