Author:
Gregory R L,Shechmeister I L,Brubaker J O,Smedberg C T,Michalek S M,McGhee J R
Abstract
Previous studies have suggested that sera from animals immunized with whole Streptococcus mutans cells may cross-react with human and monkey heart sarcolemmal tissues. In the present study, sera and saliva from rats and rabbits immunized peripherally with ribosomal preparations from S. mutans 6715 (serotype g) or GS-5 (serotype c) were examined for their ability to react with normal human heart sarcolemmal and kidney glomerular tissues by using enzyme-linked immunosorbent and immunofluorescence assays. The results showed that antibodies to serotype g and c ribosomal preparations do not react with either the human heart or renal antigens. Sera from mice immunized with human heart tissue and from a patient with a high anti-streptolysin O titer reacted strongly with human heart sarcolemmal and kidney glomerular tissues. These data indicated that ribosomal preparations from S. mutans lack the putative human heart cross-reactive determinant and suggest that the use of an S. mutans ribosomal vaccine against dental caries may not be pathogenic to human heart or renal tissues.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Reference27 articles.
1. Lack of antibodies to human heart tissue in sera of rhesus monkeys immunized with Streptococcus mutans antigens and comparative study with rabbit antisera;Bergmeier L. A.;Infect. Immun.,1983
2. Campbell D. H. J. S. Garvey N. E. Cremer and D. H. Sussdorf. 1970. Lowry (Folin-Ciocalteu) method p. 76-79. In P. Cederborg (ed.) Methods in immunology 2nd ed. W. A. Benjamin Inc. New York.
3. Reevaluation of experiments intended to demonstrate immunological crossreactions between mammalian tissues and streptococci;Christensen P.;Prog. Allergy,1979
4. Feretti J. M. Humphrey M. Begay and C. Shea. 1979. Twodimensional immunoelectrophoresis of Streptococcus mutans antigens: immunological cross-reactions p. 224-225. In M. T. Parker (ed.) Pathogenic streptococci. Reedbooks LtD. Windsor Berks England.
5. Effective immunity to dental caries: protection of gnotobiotic rats by local immunization with a ribosomal preparation from Streptococcus mutans;Gregory R. L.;Microbiol. Immunol.,1983
Cited by
4 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献