Mutations in the ilvY gene of Escherichia coli K-12 that cause constitutive expression of ilvC

Abstract

A derivative of Escherichia coli K-12 bearing an ilvC-lac fusion has been studied. beta-Galactosidase formation in this strain is under the control of the ilvC promoter and is therefore induced by the acetohydroxy acids. Derivatives of this fusion strain were isolated that constitutively expressed beta-galactosidase. When an ilvC-containing episome was introduced into these strains, acetohydroxy acid isomeroreductase was also constitutively expressed. The lesions are trans dominant and lie in ilvY, the structural gene specifying a positive control element, v, needed for induction of the isomeroreductase. It was concluded from measurements of beta-galactosidase levels in various diploid strains that, although wild-type v requires inducer to act as a positive control element, it does not act as a repressor in the absence of inducer.