Affiliation:
1. National Institute of Allergy and Infectious Diseases, U. S. Public Health Service, Bethesda, Maryland
Abstract
Repaske, Roy
(National Institute of Allergy and Infectious Diseases, Bethesda, Md.). Nutritional requirements for
Hydrogenomonas eutropha
. J. Bacteriol.
83:
418–422. 1962.—A simple apparatus for the autotrophic cultivation of
Hydrogenomonas eutropha
in 100-ml shake cultures is described. Nitrogen, in the form of ammonium, nitrate, or urea, was used for growth; nitrite could not be utilized. Optimal growth occurred at pH 6.4 to 6.8 at 30 C.
H. eutropha
grew best in an atmosphere containing 15 to 25% oxygen and 10% carbon dioxide. Below these concentrations each of the gases was limiting. Growth was shown to be dependent on iron, and the rate of growth was a function of iron concentration and its state of oxidation.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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