Use of an Inhibition Enzyme-Linked Immunosorbent Assay for Quantification of Capsular Polysaccharide or Proteins in Vaccines

Author:

Inzana Thomas J.1,Champion Anna1

Affiliation:

1. Center for Molecular Medicine and Infectious Diseases, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061

Abstract

ABSTRACT An inhibition enzyme-linked immunosorbent assay (ELISA) is described for quantification of capsular polysaccharide or proteins in vaccines and other samples containing whole cells or extracts of Actinobacillus pleuropneumoniae . The assay can be used to quantify any antigen that can be purified and for which highly specific antibodies are not available. The assay can be carried out by any laboratory capable of performing an ELISA.

Publisher

American Society for Microbiology

Subject

Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy

Reference29 articles.

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2. Anderson, P. 1978. Intrinsic tritium labeling of the capsular polysaccharide antigen of Haemophilus influenzae type b. J. Immunol.120:866-870.

3. Blackall, P. J., H. L. Klaasen, H. van den Bosch, P. Kuhnert, and J. Frey. 2002. Proposal of a new serovar of Actinobacillus pleuropneumoniae: serovar 15. Vet. Microbiol.84:47-52.

4. Humoral antibody response and protective immunity in swine following immunization with the 104-kilodalton hemolysin of Actinobacillus pleuropneumoniae

5. Frey, J. 1994. RTX-toxins in Actinobacillus pleuropneumoniae and their potential role in virulence, p. 325-340. In C. I. Kado and J. H. Crosa (ed.), Molecular mechanisms of bacterial virulence. Kluwer Academic Publishers, Amsterdam, The Netherlands.

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