Affiliation:
1. Department of Bacteriology, School of Medicine, Juntendo University, Bunkyoku, Tokyo, Japan
Abstract
Benzoate-grown cells of
Pseudomonas putida
(
arvilla
) mt-2 contain both metapyrocatechase and pyrocatechase activities, although the former activity is much higher than that of the latter. A spontaneous mutant deficient in metapyrocatechase and 2-hydroxymuconic semialdehyde hydrolyase, the first two enzymes in the
meta
-cleavage pathway of the ring of catechol, has been isolated from this strain. This mutant grows well on a minimal medium containing benzoate as a sole carbon source and has the high activity of pyrocatechase. These findings indicate that the strain mt-2 possesses the genetic capacity for enzymes of both the
meta
- and
ortho
-cleavage pathways of benzoate degradation, but its phenotypic expression is the
meta
pathway.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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