Affiliation:
1. Departments of Nutritional Sciences and Medicine, University of Wisconsin, Madison, Wisconsin 53706
Abstract
The concentration of adenosine 3′,5′-monophosphate (cyclic AMP) and the activity of adenylate cyclase were determined for the first time in conjuncation with cyclic 3′,5′-nucleotide phosphodiesterase (phosphodiesterase) during the growth cycle of
Tetrahymena pyriformis
. High levels of cyclic AMP observed during early exponential and late stationary phases were associated with elevated adenylate cyclase and decreased phosphodiesterase activities. Adenylate cyclase and cyclic AMP were decreased and phosphodiesterase was increased in cells grown in glucose-supplemented medium. In contrast to findings in mammalian liver, cyclic AMP was decreased during active gluconeogenesis in
Tetrahymena
. This suggests a different modulation of carbohydrate metabolism in the two species. The results illustrate that both the content of cyclic AMP and its action as a regulatory agent in
Tetrahymena
are uniquely suited to the metabolism of this organism.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference21 articles.
1. Adenosine-3', 5'-phosphate: identification as acrasin in a species of cellular slime mold;Barkley D. S.;Science,1969
2. An adrenergic control system in Tetrahymena;Blum J. J.;Proc. Nat. Acad. Sci. U.S.A.,1967
3. On the regulation of glycogen metabolism in Tetrahymena;Blum J. J.;Arch. Biochem. Biophys.,1970
4. The assay of adenosine 3',5'-cyclic monophosphate and guanosine 3',5'-cyclic monophosphate in biological materials by enzymatic radiosotopic displacement;Brooker G.;Biochemistry,1968
5. Adenosine 3', 5'-phosphate in biological materials. I. Purification and properties of cyclic 3', 5'-nucleotide phosphodiesterase and use of this enzyme to characterize adenosine 3', 5'-phosphate in human urine;Butcher R. W.;J. Biol. Chem.,1962
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