Identification of an Emerging Pathogen, Mycobacterium massiliense , by rpoB Sequencing of Clinical Isolates Collected in the United States

Author:

Simmon Keith E.1,Pounder June I.1,Greene John N.2,Walsh Frank3,Anderson Clint M.4,Cohen Samuel4,Petti Cathy A.145

Affiliation:

1. Associated Regional and University Pathologists (ARUP) Institute for Clinical and Experimental Pathology, Salt Lake City, Utah

2. H. Lee Moffitt Cancer and Research Institute, Tampa, Florida

3. Department of Medicine, University of South Florida College of Medicine, Tampa, Florida

4. ARUP Laboratories, Salt Lake City, Utah

5. Departments of Medicine and Pathology, University of Utah School of Medicine, Salt Lake City, Utah

Abstract

ABSTRACT Mycobacterium massiliense is a rapidly growing mycobacterium that is indistinguishable from Mycobacterium chelonae / M. abscessus by partial 16S rRNA gene sequencing. We sequenced rpoB , sodA , and hsp65 genes from isolates previously identified as being M. chelonae/M. abscessus and identified M. massiliense from isolates from two patients with invasive disease representing the first reported cases in the United States.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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